What unit are you dosing in?
Vial size
BAC water added
Desired dose
Syringe type
U100 = 100 units/mL · U40 = 40 units/mL · Standard = mL markings
Draw to Mark
40
U100 syringe markings
0 100
Concentration 5,000 mcg/ml
Draw volume 0.400 ml
Doses per vial 5

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How the Peptide Reconstitution Calculator Works

Reconstituting a peptide means adding bacteriostatic water (BAC water) to a lyophilized (freeze-dried) powder to turn it into an injectable solution. Once the peptide is dissolved, you need to know exactly how much solution to draw into your syringe to hit your target dose. That's where this calculator comes in — it takes the three variables that matter (vial size, BAC water added, desired dose) and tells you the precise syringe marking.

The math is simple arithmetic, but the unit juggling between milligrams, micrograms, milliliters, and insulin units is where mistakes happen — and a 10x dosing error with a potent peptide is not something you want to learn from experience. This calculator removes the guesswork.

The Formula (for those who want to understand)

  1. Concentration = (vial size in mcg) ÷ (BAC water in ml) = mcg per ml
  2. Draw volume (ml) = (desired dose in mcg) ÷ (concentration in mcg/ml)
  3. U100 insulin units = draw volume × 100

Example: 10 mg BPC-157 reconstituted with 2 ml BAC water has a concentration of 5,000 mcg/ml. A 250 mcg dose is 0.05 ml, or 5 units on a U100 syringe.

What Is BAC Water?

Bacteriostatic water is sterile water containing 0.9% benzyl alcohol as a preservative. The preservative suppresses bacterial growth for up to 28 days after the first puncture, which is why it's the standard diluent for multi-dose peptide vials. Plain sterile water (without preservative) is single-use only — once punctured, the clock starts ticking for microbial contamination.

BAC water is sold in 10 ml, 30 ml, and sometimes larger vials. Store unopened BAC water at room temperature away from light. Once your peptide is reconstituted, store the vial refrigerated.

Syringe Types: U100 vs U40 vs Standard

Syringe Type Units per ml 1 unit equals Best for
U100 insulin 100 0.01 ml Most peptides, small doses. US standard.
U40 insulin 40 0.025 ml Veterinary use, some international. Less common.
Standard mL Oil-based injections like testosterone (TRT).

The critical mistake to avoid: using a U40 syringe but reading the markings as if it were U100. Every "unit" on a U40 is 2.5× larger than on a U100, so the same "10 units" means a very different dose. Always confirm which syringe you're holding before drawing.

Common Peptides and Typical Doses

These are dose ranges commonly cited in published research and peptide protocol resources. They are not medical advice — always consult a qualified healthcare professional before starting any peptide protocol.

Need compound-specific guidance? We have dedicated reconstitution guides for tirzepatide, semaglutide, retatrutide, BPC-157, and GHK-Cu — each with vial-specific dose tables and titration schedules.

Peptide Typical vial size Typical dose Frequency
BPC-1575–10 mg250–500 mcg1–2× daily
TB-5005–10 mg2–5 mg2× weekly
CJC-1295 (no DAC)2–5 mg100–200 mcg1–3× daily
Ipamorelin2–5 mg100–300 mcg1–3× daily
GHK-Cu50–200 mg1–2 mgDaily (topical or injection)
Semaglutide2–10 mg0.25–2.4 mg1× weekly (titrated)
Tirzepatide10–30 mg2.5–15 mg1× weekly (titrated)
Retatrutide10–30 mg2–12 mg1× weekly (titrated)

Storage and Handling

Frequently Asked Questions

How do I calculate peptide reconstitution dosage?

Divide your desired dose (in mcg) by the concentration (peptide mcg per ml) to get the draw volume in ml. Multiply by 100 for a U100 insulin syringe to find the insulin-unit marking. This calculator does the math automatically — just pick your vial size, BAC water amount, and dose.

How much BAC water should I add to my peptide vial?

Common amounts are 1 ml, 2 ml, or 3 ml. More water means a lower concentration and larger draw volumes, which can be easier to measure precisely. Less water concentrates the solution into smaller draws. Match the water amount to the syringe size you're using and the precision you need.

What's the difference between U100 and U40 syringes?

U100 is marked so that 100 units = 1 ml (US standard). U40 is 40 units per ml, meaning each unit is 2.5× larger. Using the wrong reading on a U40 vs U100 is the #1 dosing mistake — always confirm which you have.

How long does a reconstituted peptide vial last?

Most reconstituted peptides in BAC water stay stable for around 28 days refrigerated (36–46°F). Store away from light, avoid freeze-thaw cycles, and discard if you notice cloudiness or discoloration. Some peptides like tirzepatide may remain stable longer.

Can I use this for tirzepatide, semaglutide, or retatrutide?

Yes — the math works for any reconstituted compound. Choose your unit (mg, mcg, or IU), enter your vial size and BAC water, and set your target dose. Works for BPC-157, TB-500, GHK-Cu, CJC-1295, Ipamorelin, Mod GRF, and all GLP-1 analogs.

What if my dose is in IU instead of mg?

Switch the dose unit to IU at the top of the calculator. IU is common for HCG, HGH, and insulin. Remember that IU is compound-specific — 1 IU of HCG is not the same mass as 1 IU of HGH — so always use the IU value specific to your compound.

Is this calculator free?

Yes — 100% free, no signup, no ads. For protocol scheduling, dose logging, depletion tracking, and reorder reminders, download the free Stackr iOS app.

Does this work for GLP-1 pens like Ozempic or Mounjaro?

This calculator is designed for reconstituted vials. If you use a prefilled pen (Ozempic, Wegovy, Mounjaro, Zepbound, Saxenda), you don't need to reconstitute — the dose is dialed directly on the pen. For compounded semaglutide or tirzepatide from telehealth providers like Hims, Ro, or Mochi, use this calculator since those typically ship as vials with syringes.

Want to save your calculations? The Stackr iOS app remembers your vial sizes, tracks doses, and alerts you before you run out.

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Stackr is a personal supply and protocol tracking tool. It is not intended to provide medical advice, diagnosis, or treatment. Always consult a qualified healthcare professional before starting, stopping, or changing any supplement, medication, or peptide protocol.